scRNA-Seq/sNuc-Seq technical update – Emulsion breakage step

–Protocol improvement details

Both scRNA-Seq and sNuc-Seq protocols on the Nadia Instrument?are well-established protocols resulting in outstanding gene capture rates for a large variety of different cell types. To further improve and streamline the?protocols, Dolomite Bio has replaced?the centrifugation-based emulsion break procedure with a filter-based approach.??
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The implementation of this optimized and easy-to-use step within the protocols results in reduced hands-on-time with increased, reproducible bead recovery, whilst retaining exceptional data quality.

?scRNA-Seq/sNuc-Seq protocol update

The scRNA-Seq and sNuc-Seq protocols have been updated in the highlighted area below.?

Figure 1:?The scRNA-Seq and sNuc-Seq protocols have been updated to include the filter-based emulsion breakage step as a replacement for the centrifuge-based method.?

?Workflow benefits?

–? ? ? ?Reduced hands-on time?
– Decreased number of handling steps
– Decreased number of handling steps
–? ? ? ?Streamlined bead recovery?
– Reduced risk of sample contamination and RNA-degradation
– Reduced risk of sample contamination and RNA-degradation
–? ? ? ?Exceptional data quality

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Download the updated scRNA-Seq and sNuc-Seq protocols by filling in the form below